Phone: +385 1 8886300

Email: fidelta@glpg.com

Molecular Biology

Our expertise in molecular biology covers a wide range of techniques for gene manipulation and expression analysis.

We provide:
  • Cloning and expression in prokaryotic and eukaryotic expression systems
    • Stable, transient and inducible expression by plasmid- and viral-based methodologies
    • Expression of dominant negative or constitutively active proteins (site directed mutagenesis)
    • Construction of reporter gene systems
  • RNA interference (siRNA) for target validation and assay development in desired cellular systems
  • Gene expression analysis by quantitative RT-PCR from various samples:
    • Whole blood and white blood cell subpopulations,
    • Cell lines
    • Primary cells
    • Tissues
    • Formalin fixed paraffin embedded tissue sections
  • Key equipment includes
    • Quantitative real time PCR system 7300 (Applied Biosystems)
    • Bioanalyzer 2100 (Agilent)
    • Gel imager
    • PCR instruments

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