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Wound Healing Model - Excisional Wound

The full-thickness wound in db/db mouse and in alloxan treated rats are two models of impaired wound healing in diabetes mellitus.

Delayed wound closure in db/db mice is due to impairment of wound contraction, as well as delayed formation and maturation of granulation tissue.

The rate of re-epithelialization is the same as in wild type mice.

The wide range of different read-outs permits a more precise differentiation of effects of novel therapeutic agents on the wound healing process.

Species, strain, sex:
mouse, C57bl/6; db/db, female
rat, wistar han; alloxan treated, male
Number of animals /group:
n=8-10
Pharmacological control:
becaplermin (PDGF-BB)
Routes of administration:
topical, upon request
Treatment mode:
therapeutic
Duration of dosing:   
as requested
Main read-outs:
wound closure
Facultative read-outs:
hydroxyproline, collagen
Histopathology
mRNA, IHC, morphometry
Wound closure in C57Bl/6 db/db and mice

wound area,  *p < .05, Mann-Whitney U test

Granulation tissue growth pattern in
C57Bl/6 and db/db mice


C57Bl/6 (A) and db/db (B) mice; 25X, van Gieson,
Toxicologic Pathology (2009) 37:183

References

Ševeljević-Jaran D, Čužić S, Dominis-Kramarić M, Glojnarić I, Ivetić V, Radošević S, Parnham MJ.Accelerated Healing of ExcisionalSkin Wounds by PL 14736 in Alloxan-Hyperglycemic Rats. Skin Pharmacol Appl Skin Physiol  (2006) 19:266

Tkalcević VI, Cuzić S, Brajsa K, Mildner B, Bokulić A, Situm K, Perović D, Glojnarić I, Parnham MJ. Enhancement by PL 14736 of granulation and collagen organization in healing wounds and the potential role of egr-1 expression. European Journal of Pharmacology (2007) 570:212

Ivetić Tkalčević V, Čužić S, Parnham MJ, Pašalić I, Brajša K. Differential Evaluation of Excisional Non-occluded Wound Healing in db/db Mice. Toxicologic Pathology (2009) 37:183

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