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Oxazolone Induced Chronic Delayed Type Hypersensitivity (DTH) In Mice

Chronic inflammation is induced by OXA sensitization and multiple challenges. It is characterized by Th1 and Th2 inflammatory reaction observed as increased cytokine concentration in ear tissue and IgE concentration in serum. Anti-inflammatory activity of test compounds is evaluated throughout the study (main read-outs) , while ear and other selected tissues are collected at the end of the study to be subsequently analyzed or sent to the sponsor, as requested.



Species, strain, sex:
mouse, Balb/c, male
Number of animals /group:
n=8
Pharmacological control:
Dexanethasone
Routes of administration:
topical, PO, IP, SC, IV, IM
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
upon request
Main read-outs:
ear thickness
body weight
Facultative read-outs:
inflammatory mediators in serum and ear homogenates
histopathological analysis of ear tissue
immunohistochemistry of ear tissue

Histopathological analysis of ear tissue at the end of the experiment:
a – negative control
b – vehicle
dexamethasone 

References

Ivetić Tkalčević V, Čužić S, Dominis Kramarić M, Parnham MJ, Eraković Haber V. Topical azithromycin and clarithromycin inhibit acute and chronic skin inflammation in sensitized mice, with apparent selectivity for Th2-mediated processes in delayed type hypersensitivity. Inflammation  (2011) 35:192

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