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Mouse Pneumonia Induced by Intranasal S. Pneumoniae / H. Influenzae Infection

S. pneumoniae  and H. influenzae  are the most common pathogens causing community-acquired pneumonia, a common disorder that is potentially life threatening. The aim of this model is to mimic human disease caused by S. pneumoniae or H. influenzae.

Basic study design:
0h: i.n. infection
6h: start of treatment
44-72h: sacrificing

Species, strain, sex:
mouse, C57Bl/6 or BALBc, male
Number of animals /group:
n=8-10
Pharmacological control:
azithromycin
Strain:
S.pneumoniae, H. influenzae
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
upon request
Main read-outs:
CFUs in lung tissue
Facultative read-outs:
Haematology
Serum biomarkers and inflammatory mediators
Histopathological evaluation of the lungs
Immunohistochemistry of lung tissue

References

Dominis-Kramarić M, Bosnar M, Kelnerić Ž, Glojnarić I, Čužić S, Parnham MJ and Eraković Haber V. Comparison of pulmonary inflammatory and antioxidant responses to intranasal live and heat-killed Streptococcus pneumoniae in mice. Inflammation  (2011) 34:471-486
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