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Mouse Pneumonia Induced by Intranasal Infection with ESBL Producing Klebsiella Pneumoniae

Klebsiella pneumoniae is a gram-negative opportunistic pathogen primarily infecting immuno-compromised individuals. Infections caused by this pathogen such as UTI, severe sepsis or  bacterial pneumonia can be hard to  treat due to the presence of ESBLs or carbapenemases, enzymes which confer resistance to majority of current treatment options.

The aim of this model is to mimic human disease caused by K. pneumoniae.

Basic study design:
0h: i.n. infection
1h: start of treatment
48h: sacrificing 
Species, strain, sex:
mouse, C57Bl/6, male
Number of animals /group:
n=8-10
Pharmacological control:
Meropenem
Strain:
K. pneumoniae  1470
 
(ESBL producing clinical isolate)
Treatment mode:
prophylactic
Duration of dosing:   
2 days, BID

Main read-outs:
CFUs in lung tissue

Facultative read-outs:
Haematology
Histopathological evaluation of the lungs
Immunohistochemistry of lung tissue
 
 

Models of infection with other bacteria of interest can be developed on request, using bacterial isolates from our collection or provided by the Client.

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