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LPS Induced TNF-a Production in Mice

After intraperitoneal administration, LPS, a constituent of the cell wall of Gram-negative bacteria, binds to a lipopolysaccharide binding protein (LBP) in blood. The LPS-LBP complex is a ligand for the CD14 receptor at the surface of monocytes and macrophages. The interaction of LPS-LBP with the receptor triggers the secretion of TNF-a. The anti-inflammatory activity of test compounds is evaluated 90 minutes after LPS administration (main and facultative read-outs).  

Species, strain, sex:
mouse, Balb/c, male
Number of animals /group:
n=8
Pharmacological control:
dexamethasone
Routes of administration:
PO, IP, SC, IV, IM
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
1 day or upon request

Main read-outs:
TNF-a concentration in serum

Facultative read outs:
concentration of other inflammatory  mediators in serum

LPS-induced TNF-a production in mice

 
 

References

Ivetic Tkalcevic V, Bosnjak B, Hrvacic B, Bosnar M, Marjanovic N, Ferencic Z, Situm K, Culic O, Parnham MJ, Erakovic V. Anti-inflammatory activity of azithromycin attenuates the effects of lipopolysacchyride administration in mice. Eur J Pharmacol  (2006) 539: 131

Tkalcevic VI, Bosnjak B, Pasalic I, Hrvacic B, Situm K, Kramaric MD, Glojnaric I, Haber VE. The anti-inflammatory activity of clarithromycin inhibits TNFalpha production and prolongs survival following lipopolysaccharide administration in mice. Int J Antimicrob Agents (2008) 32: 195

Ivetić Tkalčević V, Hrvačić B, Pašalić I, Eraković Haber V, Glojnarić I. Immunomodulatory effects of azithromycin on serum amyloid A production in lipopolysaccharide induced endotoxemia in mice. J Antibiot (Tokyo) (2011) 64:515

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