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LPS Induced Septic Shock in Mice

Intraplantar sensitization and intravenous challenge with LPS, a constituent of the cell wall of Gram-negative bacteria, induces production of various inflammatory mediators (TNFa, IL-12p40, CCL5, IL-10, IL-1ra) with subsequent lethal outcome of experimental animals.

The anti-inflammatory activity of test compounds is evaluated by survival monitoring (main read-out) or by measuring concentration of inflammatory mediators (facultative read-out).

Species, strain, sex:
mouse, C57Bl/6, male
Number of animals /group:
n=10 - 15
Pharmacological control:
azithromycin
Routes of administration:
PO, IP, SC, IV, IM
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
upon request

Main read-outs:
survival

Facultative read-outs:
serum inflammatory mediators

Effect of Azithromycin on survival rate in
LPS-induced septic shock



Effect of Azithromycin on serum TNF-a concentration in
LPS-induced inflammation

References

Ivetic Tkalcevic V, Bosnjak B, Hrvacic B, Bosnar M, Marjanovic N, Ferencic Z, Situm K, Culic O, Parnham MJ, Erakovic V. Anti-inflammatory activity of azithromycin attenuates the effects of lipopolysacchyride administration in mice. Eur J Pharmacol  (2006) 539: 131

Ivetić Tkalčević V, Hrvačić B, Pašalić I, Eraković Haber V, Glojnarić I. Immunomodulatory effects of azithromycin on serum amyloid A production in lipopolysaccharide induced endotoxemia in mice. J Antibiot (Tokyo) (2011) 64:515

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