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LPS Induced Pulmonary Neutrophilia in Mice

After intranasal administration LPS, a constituent of the cell wall of Gram-negative bacteria, induces production of various inflammatory mediators (CXCL1, CXCL2, TNFa, IL-6, GM-CSF, CCL2, IL-1b) and neutrophil accumulation in lung tissue.
The anti-inflammatory activity of test compounds is evaluated 24h after LPS administration  (main and facultative read-outs).
Species, strain, sex:
mouse, Balb/c, male
Number of animals /group:
n=8
Pharmacological control:
azithromycin, clarithromycin,roflumilast
Routes of administration:
IN, PO, IP, SC, IV, IM
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
immunohistochemistry
Main read-outs:
- bronchoalveolar lavage fluid (BALF) analysis
Facultative read-outs:
- inflammatory mediators in lung homogenates
- histopathology
- immunohistochemistry

 

 

References

Bosnar M, Bosnjak B, Snjezana C, Hrvacic B, Marjanovic N, Glojnaric I, Culic O, Parnham MJ, Erakovic V (2006) Azithromycin and clarithromycin inhibit lipopolysaccharide-induced murine pulmonary neutrophilia mainly through effects on macrophage-derived granulocyte-macrophage colony-stimulating factor and interleukin-1b. JPET (2006) 331: 104

Ivetic Tkalcevic V, Bosnjak B, Hrvacic B, Bosnar M, Marjanovic N, Ferencic Z, Situm K, Culic O, Parnham MJ, Erakovic V. Anti-inflammatory activity of azithromycin attenuates the effects of lipopolysacchyride administration in mice. Eur J Pharmacol (2006) 539: 131

Bosnar M, Čužić S, Bošnjak B, Nujić K, Ergović G, Marjanović N, Pašalić I, Hrvačić B, Polančec D, Glojnarić I, Eraković Haber V. Azithromycin inhibits macrophage interleukin-1β production through inhibition of activator protein-1 in lipopolysaccharide-induced murine pulmonary neutrophilia. Int Immunopharmacol  (2011) 11:424
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