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Croton Oil Induced Ear Edema in Rats

Topically administered croton oil induces Th1 inflammatory reaction manifested as vascular leakage, polymorphonuclear  infiltration and increased release of IL-1β in ear tissue. Five hours after croton oil application, ear swelling is observed.

Anti-inflammatory activity of the test compounds is evaluated by ear weighing. Ear tissue and other selected tissues can be stored for subsequent analyses.

Species, strain, sex:
rat, WH, male
Number of animals /group:
n=8
Pharmacological control:
Dexamethasone
Routes of administration:
topical, PO, IP, SC, IV, IM
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
1 day or upon request


 Main read-outs:
- ear weight

Facultative read-outs:
- inflammatory mediators in ear homogenate
- histopathology
- immunohistochemistry

Reproducibility of response to Dexamethasone

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