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Acute Tobacco Smoke Induced Pulmonary Neutrophilia in Mice

Smoking-associated COPD is characte- rized by inflammation, changes affec-ting small airways, and emphysema.
In the present model, animals are exposed to tobacco smoke for 3 days. Inflammation is assesed through analysis of BALF cell count, cytokines in the lung homogenate and lung histology. Changes affecting epithelial cells are studied in depth employing immunohistochemistry.
A recovery period can be included in the study upon request.
Species, strain, sex:
mouse, Balb/c, male
Number of animals /group:
n=8
Pharmacological control:
roflumilast
Routes of administration:
upon request
Treatment mode:
prophylactic, therapeutic
Duration of dosing:   
upon request
 Main read-outs:
 total and differential cell count in bronchoalveolar lavage fluid (BALF)
Facultative read-outs:
 - inflammatory mediators in lung homogenate
 - histopathology
 - immunohistochemistry
Effect of roflumilast on BAL cell influx in a
cute tobacco smoke pulmonary neutrophilia

Tobacco smoke induced inflammation in
lungs during a 7day recovery period

References
Čužić S, Bosnar M, Dominis Kramarić M, Ferenčić Ž, Marković D, Glojnarić I and Eraković Haber V. Claudin-3 and Clara Cell 10 kDa Protein as Early Signals of Cigarette Smoke-Induced Epithelial Injury along Alveolar Ducts. Toxicol Pathology  (2012) 40:1169
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