In vivo models of fibrosis

 

Bleomycin induced pulmonary fibrosis (mice)

Read outs:

  1. lung weight
  2. histopathology
    • Ashcroft score
    • Collagen content by Quantitative digital imaging
  3. lung function measurements (FEV, FVC)
  4. total and differential cell counts in BAL fluid
  5. gene expression
  6. hydroxyproline concentration in lung tissue homogenates
  7. IHC / IF
  8. LPA species in BALF and serum

 

Renal fibrosis (mice)
  •     Unilateral ureteral obstruction (UUO)

Read-outs: gene expression in kidneys

  •     Folic acid

Read outs: Collagen deposition by Quantitative digital imaging

 

CCl4 induced liver fibrosis (mice)

Read outs:

  1. Collagen deposition by Quantitative digital imaging
  2. Gene expression

 

CDHFD induced NASH and fibrosis (mice)

Read outs:

  1. Collagen deposition by Quantitative digital imaging
  2. Gene expression
  3. Liver enzymes
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