Compound Screening and Profiling
- Hit finding (HF)
- Hit to lead (H2L)
- Lead optimization (LO)
A number of biochemical and cellular assays in the areas of infection, inflammation and oncology have been set-up and validated in the last 15 years, addressing various target families, such as kinases, GPCRs, epigenetic targets, phospholipases, peptidases, ion channels, etc. Assays can be also customised and applied to any specific project needs.
Key equipment includes multiple liquid handling devices, robotics, multidrops and multipurpose microplate readers which enable throughput of hundreds of compounds per week in full range of concentration response curves.
- FLINT (Fluorescence Intensity)
- TRF (Time-Resolved Fluorescence)
- FP (Fluorescence Polarization/Anisotrophy)
- FRET (Fluorescence Resonance Energy Transfer)
- AlphaScreen (AlphaLISA)